Flow Cytometry Facility
The facility has 1) Two lasers and 4-colours FACS analyzer (Becton Dickinson),2) Three lasers and 8-colours FACS analyzer (Becton Dickinson),3) Four lasers and 10-colours FACS analyzer (Beckman Coulter),4) High-end, 6-ways cell sorter (Becton Dickinson).The facility is designed for cell counting, sorting, biomarker detection and protein engineering, and multiparametric analysis of the physical and/or chemical characteristics of cells routinely used for the diagnosis of health disorders, and several applications in basic research, clinical practice and clinical trials.
Mass Spectrometry Facility
The facility has AB SCIEX MALDI TOF/TOF 5800 System with Ekspert nanoLC 400 and EKSpot MALDI Spotter for comprehensive proteomics applications both qualitative and quantitative. This system facilitates the identification and analysis of the total complement of proteins and other biomolecules. The system also capable of performing to the identification of intact protein, protein/peptide identification and characterization, protein biomarker research, PTM identification and analysis, De novo sequencing, MALDI imaging, gel based and non-gel based applications, quantitative proteomics including labeled (iTRAQ, SILAC etc.) peptides, lipid and polymer analysis.
Protein expression and purification facility
The instruments available in this facility include Bioreactors, FPLC system, Cell homogenizer and High Speed Centrifuge. The facility will aid users in recombinant expression and purification of proteins at preparative and analytical grades.
"For more information, please contact Dr. Nirpendra Singh (nirpendra@rcb.res.in)."
