System and Synthetic Biology Approach for Bio-product synthesis

The Laboratory aims at using system and synthetic biology tools for developing bacterial and yeast expression systems as an ideal protein production platform. The overarching aim of laboratory are to (1) Employ metabolic engineering tools to redesign hosts for recombinant protein expression (2) Bioprocess optimization and scale-up of recombinant protein production. Currently, we intend to develop strains capable of producing advanced biofuels, bio-chemicals and therapeutically important recombinant proteins.

The other project aims at unraveling molecular mechanism and pathways behind cellulase production by fungal strain. The research focuses on aspects related to 1) Identification of novel inducer responsible for production of cellulose disintegrating enzymes 2) Understanding transcription factors involved in biomass disintegration.

• INSPIRE Faculty Award (2015-2020)
• Initiated Start up company “Butacel Bioresearch Pvt Ltd” for biochemical production
• ABLE-BEST Entrepreneurship Award

• Gupta V, Chaudhary N, Adlakha N, Gulati P, Bhatnagar R (2017). Functional analysis of BAS2108-2109 two component system: Evidence for protease regulation in Bacillus anthracis. The International Journal of Biochemistry & Cell Biology

• Pasari N, AdlakhaN,..,Yazdani SS (2017). Impact of Module-X2 and Carbohydrate Binding Module-3 on the catalytic activity of associated glycoside hydrolases towards plant biomass. Scientific Reports

• Adlakha N, Pfau T, Ebenhoeh O, Yazdani SS (2015). Insight into metabolic pathways of the potential biofuel producer, Paenibacillus polymyxa ICGEB2008. Biotechnology for Biofuels

• Adlakha N, Yazdani SS (2015). Efficient production of 2,3-butanediol from cellulosic hydrolysate using Paenibacillus sp. ICGEB2008 as a model organism. Journal of Industrial Microbiology and Biotechnology

• Bashir Z†, Kondapalli V†, Adlakha N, Sharma A, Bhatnagar R, Yazdani SS (2013). († first authorship shared) Diversity and functional significance of cellulolytic microbes living in the guts of termite, pill bug and stem borer. Scientific Reports

• Adlakha N, Ritturaj H, Rajagopal R, and Yazdani SS (2013). Draft Genome sequence of the Paenibacillus sp. ICGEB2008 (MTCC 5639) Isolated from the Gut of Helicoverpa armigera. Genome Announcement

• Gupta S, Adlakha N, Yazdani SS (2012). Efficient extracellular secretion of an endoglucanase and a β- glucosidase in E. coli. Protein Expression and Purification

• Adlakha N, Vir P and Verma I (2012). Interaction of narrow molecular mass fraction of mycobacterial secretory proteome with Type II alveolar epithelial cells. Lung India

• Adlakha N, Sawant S, Anil A, Lali A and Yazdani SS (2012). Fusion of 1,4-β-endoglucanase and 1,4-β- glucosidase enhances the cellulolytic activity and helps in channeling of the intermediates. Applied and Environmental Microbiology

• Adlakha N, Rajagopal R, Kumar S, Reddy VS and Yazdani SS (2011). Synthesis and characterization of a chimeric protein based on cellulase and xylanase enzymes from insect gut bacterium. Applied and Environmental Microbiology