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Dr. Nidhi Adlakha

Assistant Professor
E-mail: nidhi dot adlakha at rcb dot res dot in

  • PhD 2013, International Centre for Genetic Engineering and Biotechnology
  • BIRAC-BIG fellow, Technology Based Incubator, UDSC, New Delhi
  • DST-Inspire Faculty, Jawaharlal Nehru University, New Delhi

We use interdisciplinary approach including system, synthetic and molecular biology tools to develop microbial strains for industrial applications. The overarching aim of laboratory are:

  • To decipher the molecular mechanisms behind cellulase production in cellulolytic fungus.
    Recent interest towards shifting to non-food-based feedstock for biofuel production has resulted in exploitation of agricultural residues as feedstock. The filamentous fungi are exploited industrially for hydrolysis of waste agricultural residues to fermentable sugar as they possess an array of plant biomass degrading enzymes. However, the molecular mechanism underlying production of biomass degrading enzymes from cellulolytic fungi is still not known. Our laboratory is employing proteomic and transcriptional mining of cellulolytic fungi, to understand how a fungus senses and responds to different carbon sources. The knowledge gained will provide cues for improvement of fungal strains for cost effective and efficient disintegration of plant biomass into monomeric residues.

  • To Identify novel inducer responsible for production of cellulose disintegrating enzymes.
    The fungi are known as industrial workhorse for cellulase production. However, cellulase are adaptive enzymes and thus are secreted only in the presence of cellulose. On the contrary, it is well-established that complex cellulose cannot cross fungal cell wall to mediate secretion of cellulolytic enzymes. Initial study done by our group has unravelled role of few small molecules, generated from plant biomass, in inducing production of cellulase. Our group aims at performing temporal metabolomics to understand induction mechanism in different cellulolytic fungus.

  • To employ metabolic engineering tools to redesign hosts for recombinant protein expression.
    The other goal of our lab is to develop platform for improved protein production. The group is involved in bioprocess optimization, media optimization and scale-up of recombinant protein production for applications in food, feed and pharmaceutical industries.

    • INSPIRE Faculty Award (2015-2020)
    • Initiated Start up company “Butacel Bioresearch Pvt Ltd” for biochemical production
    • ABLE-BEST Entrepreneurship Award
    • Dr. Sathish Dorairaj
      Project Associate
      sathish.dorairaj@rcb.res.in

    • Sudipt Kumar Dalie
      PhD Student
      sudipt.kumar@rcb.res.in

    • Shriya Singh
      PhD Student
      shriya.singh@rcb.res.in

    • Manasa Hegde
      Project JRF
      manasa.hegde@rcb.res.in

    • Shivam Aggarwal
      MSc-PhD Integrated Student

    1. Nidhi Adlakha, Neha Munjal. (2020) Microorganisms for enhanced production of 2,3 butanediol and uses thereof . Indian Patent No.-337873

    2. Joshi H, Malik A, Aggarwal S, Munde M, Maitra SS, Adlakha N*, Bhatnagar R. (2019) In-vitro Detection of Phytopathogenic Fungal Cell Wall by Polyclonal Sera Raised Against Trimethyl Chitosan Nanoparticles. Int J Nanomedicine. doi: 10.2147/IJN.S220488 (*co-corresponding author)

    3. Gupta M, Chauhan PS, Sopory SK, Singla-Pareek SL, Pareek A, Adlakha N*, Kaur C. (2019) Draft Genome Sequence of a Potential Plant Growth-Promoting Rhizobacterium, Pseudomonas sp. Strain CK-NBRI-02.. Microbiol Resour Announc. doi: 10.1128/MRA.01113-19 (*co-corresponding author).

    4. Gupta V, Chaudhary N, Adlakha N, Gulati P, Bhatnagar R (2017). Functional analysis of BAS2108-2109 two component system: Evidence for protease regulation in Bacillus anthracis. The International Journal of Biochemistry & Cell Biology

    5. Pasari N, AdlakhaN,..,Yazdani SS (2017). Impact of Module-X2 and Carbohydrate Binding Module-3 on the catalytic activity of associated glycoside hydrolases towards plant biomass. Scientific Reports

    6. Adlakha N, Pfau T, Ebenhoeh O, Yazdani SS (2015). Insight into metabolic pathways of the potential biofuel producer, Paenibacillus polymyxa ICGEB2008. Biotechnology for Biofuels

    7. Adlakha N, Yazdani SS (2015). Efficient production of 2,3-butanediol from cellulosic hydrolysate using Paenibacillus sp. ICGEB2008 as a model organism. Journal of Industrial Microbiology and Biotechnology

    8. Bashir Z†, Kondapalli V†, Adlakha N, Sharma A, Bhatnagar R, Yazdani SS (2013). († first authorship shared) Diversity and functional significance of cellulolytic microbes living in the guts of termite, pill bug and stem borer. Scientific Reports

    9. Adlakha N, Ritturaj H, Rajagopal R, and Yazdani SS (2013). Draft Genome sequence of the Paenibacillus sp. ICGEB2008 (MTCC 5639) Isolated from the Gut of Helicoverpa armigera. Genome Announcement

    10. Gupta S, Adlakha N, Yazdani SS (2012). Efficient extracellular secretion of an endoglucanase and a β- glucosidase in E. coli. Protein Expression and Purification

    11. Adlakha N, Vir P and Verma I (2012). Interaction of narrow molecular mass fraction of mycobacterial secretory proteome with Type II alveolar epithelial cells. Lung India

    12. Adlakha N, Sawant S, Anil A, Lali A and Yazdani SS (2012). Fusion of 1,4-β-endoglucanase and 1,4-β- glucosidase enhances the cellulolytic activity and helps in channeling of the intermediates. Applied and Environmental Microbiology

    13. Adlakha N, Rajagopal R, Kumar S, Reddy VS and Yazdani SS (2011). Synthesis and characterization of a chimeric protein based on cellulase and xylanase enzymes from insect gut bacterium. Applied and Environmental Microbiology

    Dr. Nidhi Adlakha
    Regional Centre for Biotechnology
    NCR Biotech Science Cluster
    3rd Milestone, Faridabad-Gurgaon Expressway
    P.O. Box No. 3, Faridabad - 121 001
    Haryana (NCR Delhi), India
    E-mail: nidhi dot adlakha at rcb dot res dot in
    Phone: 91 129-2848977 

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