BioCARe-DBT Scientist
E-mail: babitha dot chandrashekar at rcb dot res in
Plants adapt to drought conditions by limiting water loss through stomatal regulation & prevent tissue dehydration as a survival mechanism. Stomatal regulation usually limits the uptake of CO2 and thus growth and productivity. The stomatal aperture is regulated by ion flux through channels in guard cells. The phytohormone Abscisic acid (ABA) regulates stomatal movement under water limited conditions.
We propose to regulate ABA mediated stomatal movement, but maintain ABA induced gene expression that is associated with cellular tolerance mechanisms. The emphasis is to characterize ion transporters involved in stomatal closure and opening in rice using gene editing CRISPR-Cas9 technology and chemical genomic approach. Further, a few plant E3 ligases are known to be involved in ABA induced stomatal regulation under drought. However, their precise mechanisms of regulation are not known. From this context, identifying guard cell specific E3 ligase from cell specific transcriptome data may provide leads in narrowing down the targets in regulating ABA induced stomatal movement. The goal is to provide biotechnological solutions by identifying small molecules and their utilization in agriculture to improve yield.
Dr. Babitha K Chandrashekar
Regional Centre for Biotechnology
NCR Biotech Science Cluster
3rd Milestone, Faridabad-Gurgaon Expressway
P.O. Box No. 3, Faridabad - 121 001
Haryana (NCR Delhi), India
E-mail: babitha dot chandrashekar at rcb dot res in
Phone: 91 129-2848987