RCB Logo
RCB Logo


Building
Digital India

हिन्दी / English



Dr. Prasenjit Guchhait

Professor
E-mail: prasenjit at rcb dot res dot in

  • PhD 1998, Banaras Hindu University
  • Postdoc at Baylor College of Medicine, USA
  • Instructor at Baylor College of Medicine, USA
  • Assistant Professor at Baylor College of Medicine, USA
  • Associate Professor at Regional Centre for Biotechnology, India

Major focus of Disease Biology Laboratory

  • Mechanism of protection from hypobaric hypoxia by Tibetan highlanders carrying adaptive mutations vs. development of acute mountain sickness (AMS) and high-altitude pulmonary edema (HAPE) among non-Tibetan travelers to high altitudes.
  • Effects of hyperglycemia on thrombosis and inflammation, and lung fibrosis in type-2 diabetes (T2D).
  • Prevalence of T2D among native highlanders when migrate from high altitude to sea level.
  • Effects of intravascular hemolysis on inflammation and thrombosis in hemolytic disorders including paroxysmal nocturnal hemoglobinuria (PNH) and sickle cell disease (SCD).
  • Crosstalk between complement activation and inflammation in autoimmune disorders including atypical hemolytic uremic syndrome (aHUS) and systemic lupus erythematosus (SLE).
  • Role of platelets and platelet factors in Dengue virus (DV) pathogenesis and host interferon response. Similar study in Japanese encephalitis virus (JEV) infection.
  • Understanding the mechanism of cytokine storm, lung inflammation and hypoxemia in SARS-CoV-2 infection.
  • All above studies aim to find biomarkers and molecular targets to develop potential therapeutics for prophylaxis of these complications.
  • High altitude adaptation in Tibetans and AMS/HAPE in mountain travellers: How the Tibetan specific mutation (C12G and G380C) in EGLN1 gene [which encodes Prolyl hydroxylase-2 (PHD2), or PHD2D4E/C127S variant protects them from hypoxia-triggered inflammation and thrombosis? Whether the treatment with cofactor of PHD2 such as α-Ketoglutarate (αKG) and iron (Fe2), can augment PHD2WT activity and protects mice from hypoxia-induced exaggerated inflammation/thrombosis? Whether αKG/Fe2 can be used for the prophylaxis of hypoxia-driven disorders including AMS and HAPE? Are these genetically adapted Tibetan highlanders are susceptible to infections?

    Hyperglycemia in T2D: How elevated glucose alters glycosylation of platelet proteins and activates these cells? How glucose regulates glycolysis/TCA cycle and mitochondrial function of platelets and activation of these cells? How platelet activation regulates immune cells and inflammation in mice and patients with T2D? How interaction of platelets with scar tissues of lungs and accumulation of inflammatory cells, further develop severe form of pulmonary fibrosis in T2D mice?

    High altitude adaptation and T2D: The association between genetic adaptation and phenotype such as low fatty acid oxidation and anaerobic glycolysis are known among highlanders. Does the prevalence and severity of T2D increase among native highlanders when migrate from high to low elevation? Does crosstalk among hypoxia signaling, metabolic pathways and immune systems play role in disease severity?

    Cytokine response in DV, JEV and SARS-CoV2 infections: How platelet cytokine PF4 increases DV/JEV infections and decreases interferon (IFN) signaling? How PF4 alter autophagy-lysosomal pathway and cytokine signaling of immune cells in DV infection? Do small molecule antagonists of PF4 rescue IFN signaling in monocytes and dendritic cells and protect mice from DV and JEV? Mechanism of thrombocytopenia in DV? How does lungs inflammation increases cytokine storm and causes hypoxemia (less oxygen in blood) in SARS-CoV2 infected mice/hamsters? Does the interplay between hypoxia and inflammation increase disease severity of SARS-CoV2? Whether anti-inflammatory therapy along with oxygen can rescue potentially the SARS-CoV2 pathophysiology?

    Intravascular hemolysis in PNH/SCD: How extracellular hemoglobin (Hb) activates platelets and immune cells? How Hb induces mitochondrial dysfunctions and in turn activation and apoptosis in platelets and immune cells? How crosstalk between thrombosis and inflammation develop disease severity in mice with intravascular hemolysis or patients with PNH/SCD?

    Complement activation in aHUS/SLE: How the interplay between factor-H (FH) and FH-related proteins (FHR) regulates C3 activation on immune cell surface? How complement activation develops systemic and local (organ) inflammation in aHUS/SLE? How complement activation develops hypercoagulation and thrombosis in aHUS/SLE?

    • Sulagna Bhattacharya
      Senior Research Fellow
      sulagna.sb@rcb.res.in
    • Srimali Nishith Maheshbhai
      Senior Research Fellow
      nishith@rcb.res.in
    • Sakshi Agarwal
      Junior Research Fellow
      sakshi.agarwal@rcb.res.in
    • Anamika Singh
      Junior Research Fellow
      sakshi.agarwal@rcb.res.in
    • Simrandeep Kaur
      Junior Research Fellow (Project)
      simrandeep.kaur@rcb.res.in
    1. Thakur S, Saha S, Guchhait P, Eswaran S*. (2020) Fullarazirman, nano water-soluble Fullerene C60-Mn (II) Triadduct complex with anti-cancer activity comparable to Doxorubicin. J Biomedical Nanotechnology. In Press.
    2. Sardoiwala N M, Kushwaha A, Dev A, Nishith S, Guchhait P, Karmakar S, Roy Choudhury S*. (2020) Hypericin loaded transferrin nanoparticles induce PP2A regulated BMI1 degradation in colorectal cancer specific chemo-Photodynamic Therapy. ACS Biomaterials Science & Engineering, 5, 3139-3153.
    3. Sood A, Dev A, Mohanbhai SJ, Shrimali N, Kapasiya M, Kushwaha A, Choudhury SR, Guchhait P, Karmakar S* (2019) Development and targeting efficacy of disulfide bridged chitosan-Eudragit S-100 nanoparticles for colorectal cancer. ACS Applied Nano Materials | 2, 10: 6409-17
    4. Bhasym A, Bhakuni T and Guchhait P. (2019) Elevated surface-bound complement factor-H alters the function of platelets and monocytes in FHR1/3-null healthy individuals. Blood Cells Molecules Diseases | Doi.org/10.1016/j.bcmd.2019.102349
    5. Bhasym A, Annarapu GK, Saha S, Shrimali N, Gupta S, Seth T, Guchhait P. (2019) Neutrophils develop rapid proinflammatory response after engulfing Hb-activated platelets under intravascular hemolysis. Clin Exp Immunol | Doi: 10.1111/cei.13310
    6. Bhakuni T, Singhal R, Aannarapu GK, Sharma A, Mahapatra M, Saxena R, Guchhait P (2019) Unique case of autoantibody mediated inactivation of ADAMTS13 in an Indian TTP patient. Blood Cells Molecules Diseases | 77:29-33
    7. Bhasym A, Gurjar BS, Sriharsha TM, Bhasym A, Prabhu S, Puraswani M, Khandelwal P, Saini H, Saini S, Verma AK, Chatterjee P, Bal V, George A, Sharma A, Hari P, Sinha A, Bagga A, Rath S, Guchhait P (2019) Altered Peripheral Blood Leucocyte Phenotype and Responses in Healthy Individuals with Homozygous Deletion of FHR1 and FHR3 Gene. J Clinical Immunology | Doi.org/10.1007/s10875-019-00619-2
    8. R Singhal, DK Rathore, T Bhakuni, T Seth, P Guchhait (2019) Absence of Nonclassical Monocytes in Hemolytic Patients : Free Hb and NO-Mediated Mechanism Journal of Immunology Research | doi.org/10.1155/2019/1409383
    9. Ojha A, Bhasym A, Mukherjee S, Annarapu GK, Bhakuni T, Akbar I, Seth T, Vikram NK, Vrati S, Basu A, Bhattacharyya S, Guchhait P. Platelet factor 4 promotes rapid replication and propagation of Dengue and Japanese encephalitis viruses. EBioMedicine 2018 | 39:332-347
    10. Singhal R, Chawla S, Batra H, Gupta S, Ojha A, Rathore DK, Seth T, Guchhait P (2018) Engulfment of Hb-activated platelets differentiates monocytes into pro-inflammatory macrophages in PNH patients. Eur J Immunol. [Epub ahead of print]
    11. Gurjar BS, Sriharsha TM, Bhasym A, Prabhu S, Puraswani M, Khandelwal P, Saini H, Saini S, Verma AK, Chatterjee P, Gucchait P, Bal V, George A, Rath S, Sahu A, Sharma A, Hari P, Sinha A, Bagga A. (2018) Characterization of genetic predisposition and autoantibody profile in atypical hemolytic uremic syndrome. Immunology
    12. Tashi T, Reading N S, Wuren T, Zhang X, Moore L G, Hu H, Tang F, Shestakova A, Lorenzo F, Burjanivova T, Koul P, Guchhait P, Wittwer C T, Julian C G, Shah B, Huff C D, Gordeuk V R, Prchal J T, Ge R (2017) Gain-of-function EGLN1 prolyl hydroxylase (PHD2 D4E:C127S)in combination with EPAS1 (HIF-2α) polymorphism lowershemoglobin concentration in Tibetan highlanders J Mol Med 95-665
    13. Ojha A , Nandi D, Batra H, Singhal R, Annarapu G. K., Bhattacharyya. S, Seth T, Dar L, Medigeshi G. R., Vrati S, Vikram N. K & Guchhait P (2017) Platelet activation determines the severity of thrombocytopenia in dengue infection Sci Rep 7: 41697
    14. Singhal R, Chawla S, Rathore D K, Bhasym A, Annarapu G K, Sharma V, Seth T, Guchhait P (2016)Development of pro-inflammatory phenotype in monocytes after engulfing Hb-activated platelets in hemolytic disorders.. Clinical Immunology 12: 007
    15. Annarapu GK, Singhal R, Gupta A, Chawla S, Batra H, Seth T, Guchhait P (2016) HbS Binding to GP1ba Activates Platelets in Sickle Cell Disease.. PLoS One 11:e0167899
    16. Annarapu GK, Singhal R, Peng Y, Guchhait P. (2016) Inhibition of Hb binding to GP1bα abrogates Hb-mediated thrombus formation on immobilized VWF and collagen under physiological shear stress. PLoS One. 11:e0154276
    17. Singhal R, Annarapu GK, Pandey A, Chawla S, Ojha A, Gupta A, Cruz MA, Seth T, Guchhait P. (2015)  Hemoglobin interaction with GP1ba induces platelet activation and apoptosis: a novel mechanism associated with intravascular hemolysis. Haematologica. pii: haematol.132183. [Epub ahead of print]
    18. Da Q, Teruya M, Guchhait P, Teruya J, Olson JS, Cruz MA (2015)  Free hemoglobin increases von Willebrand factor-mediated platelet adhesion in vitro: implications on circulatory devices. Blood. pii: blood-2015-05-648030. [Epub ahead of print]
    19. Pandey A, Chawla S, Guchhait P (2015)  Type-2 diabetes: Current understanding and future perspectives. IUBMB Life 67:506
    20. Lorenzo FR, Huff C, Myllymäki M, Olenchock B, Swierczek S, Tashi T, Gordeuk V, Wuran T, Li GR, McClain DL, Khan TM, Koul PA, Guchhait P, Salama ME, Xing J, Semenz GL, Liberzon E, Wilson A, Simonson TS, Jorde LB, KaelinJr WG, Koivunen P, Prchal JT. (2014) A genetic mechanism for Tibetan high-altitude adaptation. Nature Genetics 46:951.
    21. Zhou Z, Thiagarajan P, Udden MA, Lopez JA, Guchhait P. (2011) Membrane sulfatide plays a crucial role in the sickle erythrocytes adhesion to matrix and endothelial ligands. Thrombosis Haemostasis105:1046.
    22. Zhou Z, Han H, Cruz MA, Jose JA, Dong JF, Guchhait P. (2009) Hemoglobin blocks von Willebrand factor proteolysis by ADAMTS-13: a mechanism associated with sickle cell disease. Thrombosis Haemostasis 101 (6):1070.
    23. Guchhait P, Shrimpton C, Honke K, Rumbaut R, Lopez JA, Thiagarajan P. (2008) Effects of an anti-sulfatide single-chain antibody probe on platelet function. Thrombosis Haemostasis 99(3):552.

    Dr. Prasenjit Guchhait
    Professor
    Regional Centre for Biotechnology
    NCR Biotech Science Cluster
    3rd Milestone, Faridabad-Gurgaon Expressway
    P.O. Box No. 3, Faridabad - 121 001
    Haryana (NCR Delhi), India
    E-mail: prasenjit at rcb dot res dot in
    Phone: 91 129-2848821

    Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 2 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3