Building
Digital India

Dr. Prasenjit Guchhait

Professor
E-mail: prasenjit at rcb dot res dot in

  • PhD 1998, Banaras Hindu University
  • Postdoc at Baylor College of Medicine, USA
  • Instructor at Baylor College of Medicine, USA
  • Assistant Professor at Baylor College of Medicine, USA
  • Associate Professor at Regional Centre for Biotechnology, India

Our disease biology laboratory is focused on elucidatingthe complex mechanism and molecules involved in the pathogenesis of thrombosis, hypercoagulation, inflammation and impaired immune responses in different disease and stress conditions.

We have recently described-how cell-free hemoglobin (Hb) regulates the activation and functions of platelets in hemolytic disorders developing hypercoagulation and thrombosis (clot formation). We are working further to investigate the effects of Hb on inflammation and impaired immune response in patients with hemolytic disorders including aplastic anemia, PNH, HUS, thalassemia and sickle cell anemia. We are also investigatingthe differentiation and development of hematopoetic stem cells into different cell lineages under hemolytic conditions.

As an extension of our recent findings of novel mutations (C12G and G380C) in EGLN1, negative regulator of hypoxia inducible factor (HIF)1α, and their association with the decreased erythropoiesis in native Tibetans at high-altitudes, we are now investigating the association of the aboveSNPs with the immune cytome of the native highlanders. We are also investigating the procoagulant and prothrombotic protein profiles in these individuals carrying these adaptive mutations.

We are investigating the link between hypoxia responsive gene signatures and inflammasome in the pathogenesis of systemic inflammations in accidental trauma victims. Also we are investigating whether hypobaric hypoxia contributes to the progression and severity of COPD in patients at high-altitudes.

We are investigating the pathogenesis of thrombocytopenia in dengue infection with a focus on platelet-activation, platelet-antibody binding and platelet clearance. Also we are investigating the mechanism of dengue virus replication and propagation in platelets and other blood cells.

We are investigating the detail insights into the association of the homozygous deletion of the complement factor H (CFH) related1 gene (CFHR1-/-) with the immune tolerance, since 10% of healthy individuals carry this deletion and are phenotypically normal.

We are also assessing the developmental and functional aspects of neonatal immune system and their clinical consequences. More specifically, we are investigating the differences in the cord blood immune phenotypes of term appropriate for gestational age (AGAs) with more severely growth restricted small for gestational age (SGAs) neonates.

Also we are analyzing the association ofalready-reported SNPsof cytoskeletal genes such as MYH9, TUBB1 and FLNA, or integrin/glycoprotein such as ITGA2/3 and GP1b, or transcription factors including GATA1 with the giant platelet syndrome and thrombocytopenia in certain Kashmiri population.

  • Sheetal Chawla, PhD
    Young Investigator
    sheetalchawla@rcb.ac.in
  • Gowtham Kumar Annarapu
    Senior Research Fellow
    gowtham@rcb.res.in
  • Rashi Singhal
    Senior Research Fellow
    rashi@rcb.ac.in
  • Amrita Ojha
    Senior Research Fellow
    amrita.ojha@rcb.res.in
  • Angika Bhasym
    Senior Research Fellow
    angika.bhasym@rcb.res.in
  • Sulagna Bhattacharya
    Junior Research Fellow
    sulagna.sb@rcb.res.in
  • Saibal Saha
    Junior Research Fellow
    saibal@rcb.res.in
  • Sameer Gupta
    Junior Research Fellow
    sameer@rcb.res.in
  • Nidhi
    Junior Research Fellow
    nidhi@rcb.res.in
  • Srimali Nishith Maheshbhai
    Junior Research Fellow
    nishith@rcb.res.in
  1. Singhal R, Chawla S, Rathore D K, Bhasym A, Annarapu G K, Sharma V, Seth T, Guchhait P (2016) Development of pro-inflammatory phenotype in monocytes after engulfing Hb-activated platelets in hemolytic disorders.. (In Press)
  2. Annarapu GK, Singhal R, Gupta A, Chawla S, Batra H, Seth T, Guchhait P (2016) HbS Binding to GP1bα Activates Platelets in Sickle Cell Disease.. Plos One 11:e0167899
  3. Annarapu GK, Singhal R, Peng Y, Guchhait P. (2016) Inhibition of Hb binding to GP1bα abrogates Hb-mediated thrombus formation on immobilized VWF and collagen under physiological shear stress. Plos One. 11:e0154276
  4. Singhal R, Annarapu GK, Pandey A, Chawla S, Ojha A, Gupta A, Cruz MA, Seth T, Guchhait P. (2015)  Hemoglobin interaction with GP1ba induces platelet activation and apoptosis: a novel mechanism associated with intravascular hemolysis. Haematologica. pii: haematol.132183. [Epub ahead of print]
  5. Da Q, Teruya M, Guchhait P, Teruya J, Olson JS, Cruz MA (2015)  Free hemoglobin increases von Willebrand factor-mediated platelet adhesion in vitro: implications on circulatory devices. Blood. pii: blood-2015-05-648030. [Epub ahead of print]
  6. Pandey A, Chawla S, Guchhait P (2015)  Type-2 diabetes: Current understanding and future perspectives. IUBMB Life 67:506
  7. Lorenzo FR, Huff C, Myllymäki M, Olenchock B, Swierczek S, Tashi T, Gordeuk V, Wuran T, Li GR, McClain DL, Khan TM, Koul PA, Guchhait P, Salama ME, Xing J, Semenz GL, Liberzon E, Wilson A, Simonson TS, Jorde LB, Kaelin Jr WG, Koivunen P, Prchal JT. (2014) A genetic mechanism for Tibetan high-altitude adaptation. Nature Genetics 46:951.
  8. Zhou Z, Yee D, Guchhait P. (2012) Molecular link between intravascular hemolysis and vascular occlusion in sickle cell disease. Current Vascular Pharmacology 10(6):756.
  9. Zhou Z, Thiagarajan P, Udden MA, Lopez JA, Guchhait P. (2011) Membrane sulfatide plays a crucial role in the sickle erythrocytes adhesion to matrix and endothelial ligands. Thrombosis Haemostasis105:1046.
  10. Zhou Z, Behymer M, Guchhait P. (2011) Role of extracellular hemoglobin in thrombosis and vascular occlusion in patients with sickle cell anemia. Anemia 2011:918916.
  11. Zhou Z, Nguyen T, Guchhait P, Dong JF. (2010) Von Willebrand factor, ADAMTS-13, and Thrombotic thronbocytopenic purpura. Seminar in Thrombosis Hemostesis 36:71.
  12. Zhou Z, Han H, Cruz MA, Jose JA, Dong JF, Guchhait P. (2009) Hemoglobin blocks von Willebrand factor proteolysis by ADAMTS-13: a mechanism associated with sickle cell disease. Thrombosis Haemostasis 101 (6):1070.
  13. Guchhait P, Shrimpton C, Honke K, Rumbaut R, Lopez JA, Thiagarajan P. (2008) Effects of an anti-sulfatide single-chain antibody probe on platelet function. Thrombosis Haemostasis 99(3):552.
  14. Dasgupta SK, Abdel-Monem H, Guchhait P, Nagata S, Thiagarajan P. (2008) Role of lactadherin in the clearance of phosphatidylserine-expressing red blood cell in sickle cell disease. Transfusion 48(11):2370.
  15. Guchhait P, Dasgupta SK, Le A, Yellapragada S, Lopez JA, Thiagarajan P. (2007) Lactadherin mediates sickle cell adhesion to the vascular endothelial cells in flowing blood. Hematologica 92(9):1266.
  16. Dasgupta SK, Guchhait P, Thiagarajan P. (2006) Lactadherin binding and phosphatidylserine expression on cell surface-comparison with annexin A5. Translational Research 148:19.
  17. Lasser G, Guchhait P, Ellsworth J, Bishop P, Cruz MA, Lopez JA, Fruebis J. (2006) C1q-TNF related protein-1 (CTRP-1), a vascular wall protein that inhibits collagen-induced platelet aggregation by blocking VWF binding to collagen. Blood 107:423.
  18. Guchhait P, Lopez JA, Thiagarajan P. (2004) Characterization of autoantibodies against sulfatide from a V-gene phage-display library derived from patients with systemic lupus erythematosus. J Immunol Methods 295:129.
  19. Bernardo A, Bergeron AL, Sun CW, Guchhait P, Cruz MA, Lopez JA, Dong JF. (2004) Von Willebrand factor present in fibrillar collagen enhances platelet adhesion to collagen and collagen-induced platelet aggregation. J Thromb Haemost 2(4):660.
  20. Guchhait P, Tosi MF, Smith CW, Chakraborty A. (2003) The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions. J Immunol Methods 283(1-2):195.
  21. Haldar C, Singh R, Guchhait P. (2001) Relationship between the annual rhythm in melatonin and immune system in tropical palm squirrel F. pennanti Chronobiology International 18(1):61.
  22. Haldar C, Guchhait P. (2000) Pineal gland of a nocturnal bird, Indian spotted owlet A. brama: morphological and endocrine observations. J Exp Zool 287:145.
  23. Guchhait P, Haldar C. (2000) The pineal gland and adrenal influence each other in nocturnal Indian spotted owl A. brama Biogenic Amines 15(5):513.
  24. Guchhait P, Haldar C. (1999) Regulation of Pineal gland and gonadal function of a tropical nocturnal bird Indian spotted owl Athene brama following 5-methoxyindoles treatment. Biogenic Amines 15(2):263.

Dr. Prasenjit Guchhait
Associate Professor
Regional Centre for Biotechnology
NCR Biotech Science Cluster
3rd Milestone, Faridabad-Gurgaon Expressway
P.O. Box No. 3, Faridabad - 121 001
Haryana (NCR Delhi), India
E-mail: prasenjit at rcb dot res dot in
Phone: 91 129-2848821

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