RCB Logo
Building
Digital India

Dr. Prasenjit Guchhait

Professor
E-mail: prasenjit at rcb dot res dot in

  • PhD 1998, Banaras Hindu University
  • Postdoc at Baylor College of Medicine, USA
  • Instructor at Baylor College of Medicine, USA
  • Assistant Professor at Baylor College of Medicine, USA
  • Associate Professor at Regional Centre for Biotechnology, India

Our disease biology laboratory is focused on elucidatingthe complex mechanism and molecules involved in the pathogenesis of thrombosis, hypercoagulation, inflammation and impaired immune responses in different disease and stress conditions.

We have recently described-how cell-free hemoglobin (Hb) regulates the activation and functions of platelets in hemolytic disorders developing hypercoagulation and thrombosis (clot formation). We are working further to investigate the effects of Hb on inflammation and impaired immune response in patients with hemolytic disorders including aplastic anemia, PNH, HUS, thalassemia and sickle cell anemia. We are also investigatingthe differentiation and development of hematopoetic stem cells into different cell lineages under hemolytic conditions.

As an extension of our recent findings of novel mutations (C12G and G380C) in EGLN1, negative regulator of hypoxia inducible factor (HIF)1α, and their association with the decreased erythropoiesis in native Tibetans at high-altitudes, we are now investigating the association of the aboveSNPs with the immune cytome of the native highlanders. We are also investigating the procoagulant and prothrombotic protein profiles in these individuals carrying these adaptive mutations.

We are investigating the link between hypoxia responsive gene signatures and inflammasome in the pathogenesis of systemic inflammations in accidental trauma victims. Also we are investigating whether hypobaric hypoxia contributes to the progression and severity of COPD in patients at high-altitudes.

We are investigating the pathogenesis of thrombocytopenia in dengue infection with a focus on platelet-activation, platelet-antibody binding and platelet clearance. Also we are investigating the mechanism of dengue virus replication and propagation in platelets and other blood cells.

We are investigating the detail insights into the association of the homozygous deletion of the complement factor H (CFH) related1 gene (CFHR1-/-) with the immune tolerance, since 10% of healthy individuals carry this deletion and are phenotypically normal.

We are also assessing the developmental and functional aspects of neonatal immune system and their clinical consequences. More specifically, we are investigating the differences in the cord blood immune phenotypes of term appropriate for gestational age (AGAs) with more severely growth restricted small for gestational age (SGAs) neonates.

Also we are analyzing the association ofalready-reported SNPsof cytoskeletal genes such as MYH9, TUBB1 and FLNA, or integrin/glycoprotein such as ITGA2/3 and GP1b, or transcription factors including GATA1 with the giant platelet syndrome and thrombocytopenia in certain Kashmiri population.

  • Sheetal Chawla, PhD
    Young Investigator
    sheetalchawla@rcb.ac.in
  • Gowtham Kumar Annarapu
    Senior Research Fellow
    gowtham@rcb.res.in
  • Rashi Singhal
    Senior Research Fellow
    rashi@rcb.ac.in
  • Amrita Ojha
    Senior Research Fellow
    amrita.ojha@rcb.res.in
  • Angika Bhasym
    Senior Research Fellow
    angika.bhasym@rcb.res.in
  • Sulagna Bhattacharya
    Junior Research Fellow
    sulagna.sb@rcb.res.in
  • Saibal Saha
    Junior Research Fellow
    saibal@rcb.res.in
  • Sameer Gupta
    Junior Research Fellow
    sameer@rcb.res.in
  • Nidhi
    Junior Research Fellow
    nidhi@rcb.res.in
  • Srimali Nishith Maheshbhai
    Junior Research Fellow
    nishith@rcb.res.in
  1. Tashi T, Reading N S, Wuren T, Zhang X, Moore L G, Hu H, Tang F, Shestakova A, Lorenzo F, Burjanivova T, Koul P, Guchhait P, Wittwer C T, Julian C G, Shah B, Huff C D, Gordeuk V R, Prchal J T, Ge R (2017) Gain-of-function EGLN1 prolyl hydroxylase (PHD2 D4E:C127S)in combination with EPAS1 (HIF-2α) polymorphism lowershemoglobin concentration in Tibetan highlanders J Mol Med pp 1-6
  2. Ojha A , Nandi D, Batra H, Singhal R, Annarapu G. K., Bhattacharyya. S, Seth T, Dar L, Medigeshi G. R., Vrati S, Vikram N. K & Guchhait P (2017) Platelet activation determines the severity of thrombocytopenia in dengue infection Sci Rep 7: 41697
  3. Singhal R, Chawla S, Rathore D K, Bhasym A, Annarapu G K, Sharma V, Seth T, Guchhait P (2016)Development of pro-inflammatory phenotype in monocytes after engulfing Hb-activated platelets in hemolytic disorders.. Clinical Immunology 12: 007
  4. Annarapu GK, Singhal R, Gupta A, Chawla S, Batra H, Seth T, Guchhait P (2016) HbS Binding to GP1ba Activates Platelets in Sickle Cell Disease.. PLoS One 11:e0167899
  5. Annarapu GK, Singhal R, Peng Y, Guchhait P. (2016) Inhibition of Hb binding to GP1bα abrogates Hb-mediated thrombus formation on immobilized VWF and collagen under physiological shear stress. PLoS One. 11:e0154276
  6. Singhal R, Annarapu GK, Pandey A, Chawla S, Ojha A, Gupta A, Cruz MA, Seth T, Guchhait P. (2015)  Hemoglobin interaction with GP1ba induces platelet activation and apoptosis: a novel mechanism associated with intravascular hemolysis. Haematologica. pii: haematol.132183. [Epub ahead of print]
  7. Da Q, Teruya M, Guchhait P, Teruya J, Olson JS, Cruz MA (2015)  Free hemoglobin increases von Willebrand factor-mediated platelet adhesion in vitro: implications on circulatory devices. Blood. pii: blood-2015-05-648030. [Epub ahead of print]
  8. Pandey A, Chawla S, Guchhait P (2015)  Type-2 diabetes: Current understanding and future perspectives. IUBMB Life 67:506
  9. Lorenzo FR, Huff C, Myllymäki M, Olenchock B, Swierczek S, Tashi T, Gordeuk V, Wuran T, Li GR, McClain DL, Khan TM, Koul PA, Guchhait P, Salama ME, Xing J, Semenz GL, Liberzon E, Wilson A, Simonson TS, Jorde LB, KaelinJr WG, Koivunen P, Prchal JT. (2014) A genetic mechanism for Tibetan high-altitude adaptation. Nature Genetics 46:951.
  10. Zhou Z, Thiagarajan P, Udden MA, Lopez JA, Guchhait P. (2011) Membrane sulfatide plays a crucial role in the sickle erythrocytes adhesion to matrix and endothelial ligands. Thrombosis Haemostasis105:1046.
  11. Zhou Z, Han H, Cruz MA, Jose JA, Dong JF, Guchhait P. (2009) Hemoglobin blocks von Willebrand factor proteolysis by ADAMTS-13: a mechanism associated with sickle cell disease. Thrombosis Haemostasis 101 (6):1070.
  12. Guchhait P, Shrimpton C, Honke K, Rumbaut R, Lopez JA, Thiagarajan P. (2008) Effects of an anti-sulfatide single-chain antibody probe on platelet function. Thrombosis Haemostasis 99(3):552.

Dr. Prasenjit Guchhait
Associate Professor
Regional Centre for Biotechnology
NCR Biotech Science Cluster
3rd Milestone, Faridabad-Gurgaon Expressway
P.O. Box No. 3, Faridabad - 121 001
Haryana (NCR Delhi), India
E-mail: prasenjit at rcb dot res dot in
Phone: 91 129-2848821

Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 2 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3